Composite

Part:BBa_K2060000

Designed by: Cardiff_Wales Team, Geraint Parry   Group: iGEM16_Cardiff_Wales   (2016-09-23)


CRISPR-Cas9 guide RNA targeting to 16S RNA

This part includes a guide RNA that will target Cas9 to the E.Coli 16S ribosomal RNA locus. The guide RNA is expressed with the Cas9-recognised RNA scaffold. Expression of the guideRNA is driven by the T7 promotor with Lac0 binding site and followed by a T7 terminator sequence.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 48
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None